EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF KADPAAYAN (PSYCHOTRIA LUZONENSIS) IN EGG WHITE INDUCED INFLAMMATION IN ALBINO RATS

Abstract

Inflammation is a vital part of the body’s immune response. Without inflammation, wounds would fester, and infections could become deadly. This study focused on the evaluating the anti-inflammatory activity of Kadpaayan (Psychotria luzoniensis) in different concentrations of the leaf extract: 100%, 75%, 50%, and 25%, with the used of egg white- induced rat paw edema. A plant sample was collected at Tawi, Penablanca, Cagayan, around 7:00-9:00am in the morning. Leaves were washed and air-dried for 7 days at room temperature. The dried leaves were macerated using 70% diluted alcohol for 72 hours. After filtering extracts and the evaporation of solvent, they undergo phytochemical screening, where the secondary metabolites present are coumarins, saponins, and phenols, which exhibit anti- inflammatory activity. The prepared concentration was compared to the positive control (Diclofenac), an anti-inflammatory drug available in the market. A total of thirty (30) albino rats of both sexes were selected in this study, with six (6) treatments having five (5) replicates each. To induce inflammation, 0.02 ml of egg white were injected into each of the rats’ paws, and water volume displacement were used as a method. The concentration treatment and standard drug were administered orally after thirty (30) minutes of induction. Data showed that all concentrations have potential for anti-inflammatory activity, but 25% and 100% concentrations exhibited the greatest anti-inflammatory activity, as inferred from the progressively lower displacement values. The results showed that there is no significant difference in the anti-inflammatory activity of different concentrations of Kadpaayan (Psychotria luzoniensis) leaf extract and the positive control. Therefore, it concludes that Kadpaayan leaf extract is comparable in its ability to prevent inflammation against egg white-induced paw edema in albino rats.